RESOLUTION IN AUTORADIOGRAPHY USING SEMITHIN SECTIONS
نویسندگان
چکیده
منابع مشابه
Electron microscopy of synaptonemal complexes in semithin sections.
The technique of osmium tetroxide fixation followed by a treatment with p-phenylenediamine was applied to mouse testes in order to analyze pachytene nuclei. Observation of Epon semithin sections (0.25--0.5 micrometer) in conventional electron microscopy revealed the presence of positively stained synaptonemal complexes surrounded by unstained chromatin. The present results show that by using th...
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Immunostaining of semithin sections is a valuable tool in biomedical research; however, this method is rather rarely used by histologists. To overcome the apparent reservations concerning this method, the present report describes a technique which is rather simple, largely standardized, and very useful in investigative endocrinology. The technique includes the following steps: snap-freezing and...
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The sensitivity of monolayers of Ilford L-4 nuclear emulsion to beta(-)-particles is approximately 0.20 grain per particle emitted for H(3), and 0.025 grain per particle for p(32). The sensitivity for thick layers is 1.3 grains per tritium beta(-)-particle emitted within the emulsion. In electron microscopic autoradiographs the maximum resolution is approximately 0.1 micron for H(3) and 0.3 mic...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1974
ISSN: 0022-1554,1551-5044
DOI: 10.1177/22.4.217